| Accession Number | DEG10340106 |
| Gene name | dnaE |
| Gene Ref | 375357046 |
| RefSeq | NC_016776 |
| Function Class | - |
| Description | putative DNA polymerase III alpha subunit |
| Uniprot accession | E1WLA4 |
| Organism | Bacteroides fragilis 638R |
| Condition | Rich medium |
| Uniprot accession | E1WLA4 |
| Operon ID in DOOR database | 1212301 |
| COG | - |
| Location | 825305..829189(+); lagging strand |
| Reference | Yaligara Veeranagouda, et al (2014). "Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library". BMC Genomics, 15:429. |
| Nucleotide sequence |
Length: 3885 nt ATGACTTATGTTGATAACTTTTTGATGTTTATCTTTGTTAGTAGCTGTAGACATTGTAATTTTATGGCTTCAAATTATCAACCTATAACTATGCAGGATTTCGTTCATTTACACGTCCATACGCAATATTCTCTTCTGGATGGTCAGGCCAGTGTCAGTGCATTGGTCGATAAGGCTATGAAAGACGGGATGAAAGGTATTGCCGTTACGGACCATGGAAACATGTTCGCCATTAAAGAGTTTACGAACTATGTTAATAAGAAAAATGGAGGTCCGAAAGGAGAAATAAAGGACCTGAAGAAGCGGATTGCAGCTATTGAAGCCGGTGAAGTGGAGTGTGCGGATAAAGATGCCGAGATTGCTGACTGTAAAGCTAAAATAGCGGATGCGGAAGGGCGATTGTTCAAGCCTATCATAGGCTGTGAGATGTATGTGGCTCGCCGTACAATGGATAAAAAGGAGGGAAAGCCTGACCAGAGCGGGTATCACCTGATTGTGCTCGCCAAAAACGAGAAGGGGTATCATAACCTGATAAAATTGGTTTCGCGGGCATGGACCAAAGGTTACTATATGCGTCCCCGTACAGACCGGAACGAGTTGGAGAAATATCACGAAGGTTTGATTATCTGTTCTGCGTGTTTGGGCGGTGAAGTGCCCAAAAAGATAACTCAGGGATTGTTGGCGGAAGCAGAAGAAGCCATCCAATGGTATAAGAACCTGTTTGGAGACGATTATTATCTGGAAATGCAGCGCCATAAAGCGACAGTGCCTAAAGCAAATCATGAAGCTTATCCGTTGCAGGTGAATGTAAATAAGCATTTGATTGAATACTCCAAAAAGTATAATGTCAAGTTAATCTGTACGAATGATGTTCACTTTGTCAACGAAGAACATGCTGAGGCGCACGATCGGCTGATCTGTTTGAGTACGGGAAAAGATCTCGACGATCCGAACCGGATGTATTATACGAAACAAGAGTGGATGAAGACGAAGGCAGAGATGAATGAACTCTTTGCCGATGTGCCCGAAGCATTGAGTAATACACTTGAGATTCTGGATAAAGTAGAGTATTATTCAATCGATCACGCACCGATTATGCCTACTTTTGCAATCCCCGAGGACTTTGGAACAGAAGAAGGATATCGGCAAAAATATACGGAAAAGGATCTTTTTGATGAGTTCACCCAAGACGAAAACGGCAACGTGGTGTTGAGTGAGGAAGCAGCCAAAGATAAAATAAAACGTTTGGGAGGCTATGATAAACTGTACCGTATCAAACTGGAAGCCGACTATCTGAAAAAGCTGACTTTCGACGGAGCTAAGAAGTTTTATGGCGATCCGTTGTCACCGGAGGTCAAAGAGCGGCTGGTCTTTGAATTACATATTATGAAGACCATGGGTTTCCCGGGATACTTTCTTATAGTACAAGATTTTATTGCCGCCGGGCGTAATATGGGAGTCTCCATTGGTCCGGGACGTGGTTCCGCTGCCGGTTCTGCCGTAGCCTATTGTTTGCAGATCACTAAAATAGACCCGATCAAATACGATTTGCTGTTTGAGCGTTTCTTGAATCCCGATCGTATTTCATTGCCTGATATCGATATTGACTTCGATGATGATGGTCGTGGCGAAGTGTTACGTTGGGTGACGGAGAAATATGGACAGGAAAAGGTGGCGCATATCATTACCTATGGTACTATGGCTACGAAACTGGCTATCAAAGATGTTGCCCGTGTCCAGAAACTTCCGCTTGCCGAATCGGATCGCTTGGCCAAATTGGTTCCGGATAAAATTCCGGATAAGAAACTGAATCTGAAGAATGCCATAGAATATGTGCCCGAATTGCAGGCGGCTGAAGCATCTCCCGATCCTTTGGTGAGGGATACGATGAAATATGCCAAGATGCTTGAGGGGAATGTGCGTGGAACGGGTGTACATGCCTGTGGCACTATTATTTGTCGTGATGATATCACGGATTGGGTACCGGTCAGCACGGCTGATGATAAAGAGACCGGCGAAAAGATGCTGGTTACCCAATATGAAGGTTCGGTGATCGAAGATACCGGATTGATCAAGATGGACTTTCTGGGGCTGAAAACATTGTCTATTATAAAGGAGGCTGTCGAAAATATTCGTTTGAGTAAAGGAATGGAACTGGATATCGATTCCATTTCAATCGAGGATCCGGCTACTTATAAACTTTACAGTGACGGACGAACGATCGGTACTTTCCAGTTTGAGTCTGCCGGTATGCAGAAGTACCTGCGTGAGTTGCAACCTTCTACGTTTGAGGATCTGATTGCGATGAATGCCCTTTACCGTCCGGGTCCGATGGATTATATTCCTGACTTTATTGATCGTAAACATGGACGTAAGCCTATTGAATATGATATTCCTGTCATGGAGAAATACCTGAAGGATACATACGGTATTACGGTCTATCAGGAGCAGGTCATGCTTTTGTCACGTCTGCTGGCCGACTTTACGCGTGGTGAATCGGATGCCCTCCGTAAAGCGATGGGTAAAAAATTGCGTGATAAGTTGGATCACATGAAACCCAAGTTTGTAGAAGGCGGAAGGAAAAACGGACACGACCCGAAAGTTCTTGAAAAGATTTGGGCAGACTGGGAGAAATTTGCATCGTATGCGTTCAATAAATCACATGCCACCTGTTATTCTTGGGTTGCCTATCAGACTGCTTATCTGAAAGCCAATTATCCGGCTGAATATATGGCTGCTGTCATGAGCCGAAGTTTGTCGAATATCACTGATATTACCAAACTGATGGACGAGTGTAAAATGATGGGAGTACAGACGTTGGGACCGGATGTGAATGAAAGTAACCTGAAGTTTACCGTAAACCGGAATGGAAATATACGTTTCGGATTGGGAGCCGTCAAAGGTGTGGGTGAGGCTGCCGTGCAAAGCATCATGGAGGAGCGAAAAGAGAACGGTCCCTTTAAAGGTATCTTCGACTTTGTGCAGCGTGTCAACCTGAATGCTTGTAACAAGAAAAATATGGAATGCCTGGCACTTGCCGGTGGATTCGATAGCTTTCCTGAACTGAAGCGTGAACAGTATTTTGCTGTGAACAGCAAAGGAGAGACTTTTCTTGAGACATTGATGCGTTATGGCAACCGTTACCAAGCTGATAAGGCTGCCGCCGTTAATTCTTTGTTCGGCGGAGATAACGTTATCGATATAGCGACTCCCGAAATTCTACCGGCGGAACGTTGGAACGATTTGGAGAGACTGAATAAGGAACGCGAACTGGTAGGTATTTATTTGTCGGCACATCCGCTTGATGAATATGCCATTGTGTTGGAACATGTTTGCAATACACATATGAGCGAGCTTGATGATAAATCGGCTTTGGCAGGAAGAGAGATTACGATGGGAGGCATTGTTACCAGTGTCCGCCGGGGTATCAGTAAGAATGGAAATCCTTATGGTATAGCTAAAATAGAAGATTATTCCGGTTCGGCAGAGATTCCTTTCTTTGGAAATGACTGGGTTACTTTCCAAGGCTATTTAGGTGAAGGTACTTTCTTGTTTATCAAAGCCCGTTGTCAACCTAAACAATGGCGTCCCGATGAACTTGATGTCAAGATTACTTCGATGGAATTGCTGCCCGATGTCAAGGAACAACTGATAGAGAAGATTACGATTCTGATTCCGCTTGCCGAACTTAATTCGGCTTTGGTAACCGAGCTTGCTTCTTTAACGAAAGAACACCCGGGAAATACGGAACTTTATTTCAAAGTAACGGATACGGAGGGGAAGATGTATGTAGACCTGATTTCCCGTCCTGTGAAACTTTCGGTTGGCAGAGAATTAATTTCCTATTTAAAGGAACGTCCCGAACTTGCGTTTCATATAAATTGA |
| Amino acid sequence |
Length: 1294 aa MTYVDNFLMFIFVSSCRHCNFMASNYQPITMQDFVHLHVHTQYSLLDGQASVSALVDKAMKDGMKGIAVTDHGNMFAIKEFTNYVNKKNGGPKGEIKDLKKRIAAIEAGEVECADKDAEIADCKAKIADAEGRLFKPIIGCEMYVARRTMDKKEGKPDQSGYHLIVLAKNEKGYHNLIKLVSRAWTKGYYMRPRTDRNELEKYHEGLIICSACLGGEVPKKITQGLLAEAEEAIQWYKNLFGDDYYLEMQRHKATVPKANHEAYPLQVNVNKHLIEYSKKYNVKLICTNDVHFVNEEHAEAHDRLICLSTGKDLDDPNRMYYTKQEWMKTKAEMNELFADVPEALSNTLEILDKVEYYSIDHAPIMPTFAIPEDFGTEEGYRQKYTEKDLFDEFTQDENGNVVLSEEAAKDKIKRLGGYDKLYRIKLEADYLKKLTFDGAKKFYGDPLSPEVKERLVFELHIMKTMGFPGYFLIVQDFIAAGRNMGVSIGPGRGSAAGSAVAYCLQITKIDPIKYDLLFERFLNPDRISLPDIDIDFDDDGRGEVLRWVTEKYGQEKVAHIITYGTMATKLAIKDVARVQKLPLAESDRLAKLVPDKIPDKKLNLKNAIEYVPELQAAEASPDPLVRDTMKYAKMLEGNVRGTGVHACGTIICRDDITDWVPVSTADDKETGEKMLVTQYEGSVIEDTGLIKMDFLGLKTLSIIKEAVENIRLSKGMELDIDSISIEDPATYKLYSDGRTIGTFQFESAGMQKYLRELQPSTFEDLIAMNALYRPGPMDYIPDFIDRKHGRKPIEYDIPVMEKYLKDTYGITVYQEQVMLLSRLLADFTRGESDALRKAMGKKLRDKLDHMKPKFVEGGRKNGHDPKVLEKIWADWEKFASYAFNKSHATCYSWVAYQTAYLKANYPAEYMAAVMSRSLSNITDITKLMDECKMMGVQTLGPDVNESNLKFTVNRNGNIRFGLGAVKGVGEAAVQSIMEERKENGPFKGIFDFVQRVNLNACNKKNMECLALAGGFDSFPELKREQYFAVNSKGETFLETLMRYGNRYQADKAAAVNSLFGGDNVIDIATPEILPAERWNDLERLNKERELVGIYLSAHPLDEYAIVLEHVCNTHMSELDDKSALAGREITMGGIVTSVRRGISKNGNPYGIAKIEDYSGSAEIPFFGNDWVTFQGYLGEGTFLFIKARCQPKQWRPDELDVKITSMELLPDVKEQLIEKITILIPLAELNSALVTELASLTKEHPGNTELYFKVTDTEGKMYVDLISRPVKLSVGRELISYLKERPELAFHIN |