| No. | Organism | Essential genes | Conditions | Reference |
|---|---|---|---|---|
| 1 | Bacillus subtilis 168 | 271 | Rich medium | Kobayashi K, et al (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A, 100:4678-83. |
| 2 | Staphylococcus aureus N315 | 302 | Rich medium | Ji Y, et al (2001). Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science, 293:2266-9. |
| 3 | Vibrio cholerae N16961 | 779 | Rich medium | Cameron DE, Urbach MJ,Mekalanos JJ(2008). A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae. PNAS, 105(25):8736-8741. |
| 4 | Haemophilus influenzae Rd KW20 | 642 | Rich medium | Akerley BJ, et al (2002). A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A, 99:966-71. |
| 5 | Mycoplasma genitalium G37 | 381 | Rich medium | Glass JI, et al (2006). Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A, 103:425-30. |
| 6 | Streptococcus pneumoniae | 244 | Rich medium | Thanassi JA, et al (2002). Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res, 30:3152-62. |
| 7 | Helicobacter pylori 26695 | 323 | Rich medium | Salama NR, et al (2004). Global transposon mutagenesis and essential gene analysis of Helicobacter pylori. J Bacteriol, 186:7926-35. |
| 8 | Mycobacterium tuberculosis H37Rv | 614 | Rich medium | Sassetti CM, et al (2003). Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 48:77-84. |
| 9 | Salmonella typhimurium LT2 | 230 | Rich medium | Knuth K, et al (2004). Large-scale identification of essential Salmonella genes by trapping lethal insertions. Mol Microbiol, 51:1729-44. |
| 10 | Francisella novicida U112 | 392 | Rich medium | Gallagher LA, et al (2007). A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A, 104:1009-14. |
| 11 | Acinetobacter baylyi ADP1 | 499 | Rich medium | de Berardinis V, et al (2008). A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1. Mol Syst Biol. |
| 12 | Mycoplasma pulmonis UAB CTIP | 310 | Rich medium | French CT, et al (2008). Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol. |
| 13 | Pseudomonas aeruginosa UCBPP-PA14 | 335 | Rich medium | Liberati NT, et al (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A, 103:2833-8. |
| 14 | Salmonella enterica serovar Typhi | 353 | Rich medium; Required for Bile acid tolerance |
Langridge GC, et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res, 19:2308-16. |
| 15 | Staphylococcus aureus NCTC 8325 | 351 | Rich medium | Chaudhuri RR, et al (2009). Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH). BMC Genomics, 10:291. |
| 16 | Escherichia coli MG1655 I | 609 | Rich medium | Gerdes SY, et al (2003). Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 185:5673-84.['Supplemental Materials'] |
| 17 | Escherichia coli MG1655 II | 296 | Rich medium | Baba T, et al (2006). Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. |
| 18 | Caulobacter crescentus | 480 | Rich medium | Christen B, et al (2011). The essential genome of a bacterium. Mol Syst Biol, 7:528. |
| 19 | Streptococcus sanguinis | 218 | Rich medium | Xu, P et al(2011). Genome-wide essential gene identification in Streptococcus sanguinis. Sci. Rep. 1, 125; DOI:10.1038/srep00125. |
| 20 | Porphyromonas gingivalis ATCC 33277 | 463 | Rich medium | Klein BA, et al (2012). Identification of essential genes of the periodontal pathogen Porphyromonas. BMC Genomics, 13: 578. |
| 21 | Bacteroides thetaiotaomicron VPI-5482 | 325 | Rich medium | Goodman Al, et al (2009). Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat. Cell Host & Microbe, 6: 279-289. |
| 22 | Burkholderia thailandensis E264 | 406 | Rich medium. | Baugh, Loren, et al. "Combining Functional and Structural Genomics to Sample the Essential Burkholderia Structome." PloS one 8.1 (2013): e53851. |
| 23 | Mycobacterium tuberculosis H37Rv II | 771 | Rich medium; Required for cholesterol metabolism |
Griffin, Jennifer E., et al. "High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism." PLoS pathogens 7.9 (2011): e1002251. |
| 24 | Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S | 105 | Rich medium; Required for Bile acid tolerance |
Khatiwara, Anita, et al. "Genome scanning for conditionally essential genes in salmonella enterica serotype typhimurium." Applied and environmental microbiology 78.9 (2012): 3098-3107. |
| 25 | Mycobacterium tuberculosis H37Rv III | 687 | Rich medium | Zhang, Yanjia J., et al. "Global assessment of genomic regions required for growth in Mycobacterium tuberculosis." PLoS pathogens 8.9 (2012): e1002946. |
| 26 | Sphingomonas wittichii RW1 | 535 | Rich medium | Roggo, Clemence, et al. "Genome-wide transposon insertion scanning of environmental survival functions in the polycyclic aromatic hydrocarbon degrading bacterium Sphingomonas wittichii RW1." Environmental microbiology (2013). |
| 27 | Shewanella oneidensis MR-1 | 403 | Rich medium | Deutschbauer, Adam, et al. "Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions." PLoS genetics 7.11 (2011): e1002385. |
| 28 | Pseudomonas aeruginosa PAO1 | 117 | Tobramycin and Rich medium | Gallagher, Larry A., Jay Shendure, and Colin Manoil. "Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq." MBio 2.1 (2011). |
| 29 | Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 | 228 | Rich medium | Metris, Aline, et al. "In vivo and in silico determination of essential genes of Campylobacter jejuni." BMC genomics 12.1 (2011): 535. |
| 30 | Salmonella enterica serovar Typhimurium SL1344 | 353 | Rich medium | Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. |
| 31 | Salmonella enterica serovar Typhi Ty2 | 358 | Rich medium | Barquist, Lars, et al. "A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium." Nucleic acids research 41.8 (2013): 4549-4564. |
| 32 | Bacteroides fragilis 638R | 547 | Rich medium. | Yaligara Veeranagouda, et al (2014). "Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library". BMC Genomics, 15:429. |
| 33 | Burkholderia pseudomallei K96243 | 505 | Rich medium | Moule, M.G., et al. (2014) Genome-Wide Saturation Mutagenesis of Burkholderia pseudomallei K96243 Predicts Essential Genes and Novel Targets for Antimicrobial Development. MBio 5, e00926-00913 |
| 34 | Pseudomonas aeruginosa PAO1 | 336 | Rich medium | Turner, K.H., Wessel, A.K., Palmer, G.C., Murray, J.L. and Whiteley, M. (2015) Essential genome of Pseudomonas aeruginosa in cystic fibrosis sputum. Proc. Natl. Acad. Sci. U. S. A. |
| 35 | Streptococcus pyogenes MGAS5448 | 227 | Todd-Hewitt medium | Le Breton Y. et al (2015). Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes. Sci. Rep. 5, 9838. |
| 36 | Streptococcus pyogenes NZ131 | 241 | Todd-Hewitt medium | Le Breton Y. et al (2015). Essential Genes in the Core Genome of the Human Pathogen Streptococcus pyogenes. Sci. Rep. 5, 9838. |
| 37 | Porphyromonas gingivalis ATCC 33277 | 281 | Rich medium | Hutcherson, Justin A., et al (2015). Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon sequencing libraries. Molecular Oral Microbiology. |
| 38 | Synechococcus elongatus PCC 7942 | 682 | Rich medium | Rubin, Benjamin E., et al. "The essential gene set of a photosynthetic organism." Proceedings of the National Academy of Sciences (2015): 201519220. |
| 39 | Rhodopseudomonas palustris CGA009 | 522 | Rich medium | Pechter, Kieran B., et al. "The essential genome of the metabolically versatile alphaproteobacterium Rhodopseudomonas palustris." Journal of bacteriology (2015): JB-00771. |
| 40 | Streptococcus agalactiae A909 | 317 | Rich medium | Hooven, T.A. et al. The Essential Genome of Streptococcus Agalactiae.BMC Genomics, 17, 406, 2016. |
| 41 | Acinetobacter baumannii ATCC 17978 | 458 | Rich medium | Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. |
| 42 | Acinetobacter baumannii ATCC 17978 | 156 | Murine model of pneumonia | Wang, N., et al. (2014) Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung, MBio, 5, e01163-01114. |
| 43 | Agrobacterium fabrum str. C58 | 361 | Rich medium | Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. |
| 44 | Brevundimonas subvibrioides ATCC 15264 | 412 | Rich medium | Curtis, P.D. and Brun, Y.V. (2014) Identification of essential alphaproteobacterial genes reveals operational variability in conserved developmental and cell cycle systems, Mol. Microbiol., 93, 713-735. |
| 45 | Bacillus thuringiensis BMB171 | 516 | Rich medium | Bishop, A.H., Rachwal, P.A. and Vaid, A. (2014) Identification of genes required by Bacillus thuringiensis for survival in soil by transposon-directed insertion site sequencing, Curr. Microbiol., 68, 477-485. |
| 46 | Escherichia coli ST131 strain EC958 | 315 | Rich medium with appropriate antibiotics | Phan, M.D., et al. (2013) The serum resistome of a globally disseminated multidrug resistant uropathogenic Escherichia coli clone, PLoS Genet., 9, e1003834. |
| 47 | Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 | 166 | Rich medium; optimal growth on MH agar plates under microaerophilic conditions at 37°C | Mandal, R. K., Jiang, T., & Kwon, Y. M. (2017). Essential genome of Campylobacter jejuni. BMC genomics, 18(1), 616. |
| 48 | Campylobacter jejuni subsp. jejuni 81-176 | 384 | Rich medium; optimal growth on MH agar plates under microaerophilic conditions at 37°C | Mandal, R. K., Jiang, T., & Kwon, Y. M. (2017). Essential genome of Campylobacter jejuni. BMC genomics, 18(1), 616. |
| 49 | Pseudomonas aeruginosa PAO1 | 551 | LB agar, MOPS-pyruvate agar and Cystic fibrosis sputum agar | Lee, S. A., Gallagher, L. A., Thongdee, M., Staudinger, B. J., Lippman, S., Singh, P. K., & Manoil, C. (2015). General and condition-specific essential functions of Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences, 112(16), 5189-5194. |
| 50 | Francisella tularensis Schu S4 | 453 | Rich medium; kanamycin (10 g/ml), chloramphenicol (5 g/ml), or polymyxin B (100 g/ml) | Ireland, P. M., et al. (2019). Global analysis of genes essential for Francisella tularensis Schu S4 growth in vitro and for fitness during competitive infection of Fischer 344 rats. Journal of bacteriology, 201(7), e00630-18. |
| 51 | Burkholderia cenocepacia K56-2 | 508 | Rich medium; Luria-Bertani (LB) media | Gislason, A. S., Turner, K., Domaratzki, M., & Cardona, S. T. (2017). Comparative analysis of the Burkholderia cenocepacia K56-2 essential genome reveals cell envelope functions that are uniquely required for survival in species of the genus Burkholderia. Microbial genomics, 3(11). |
| 52 | Streptococcus mutans UA159 | 197 | Rich medium; blood agar | Shields, R. C., Zeng, L., Culp, D. J., & Burne, R. A. (2018). Genomewide identification of essential genes and fitness determinants of Streptococcus mutans UA159. MSphere, 3(1), e00031-18. |
| 53 | Neisseria gonorrhoeae MS11 | 751 | Rich medium; GC agar | Remmele, C. W., Xian, Y., Albrecht, M., Faulstich, M., Fraunholz, M., Heinrichs, E., ... & Rudel, T. (2014). Transcriptional landscape and essential genes of Neisseria gonorrhoeae. Nucleic acids research, 42(16), 10579-10595. |
| 54 | Escherichia coli O157:H7 | 1265 | Rich medium; LB agar | Warr, A. R., Hubbard, T. P., Munera, D., Blondel, C. J., Zur Wiesch, P. A., Abel, S., ... & Waldor, M. K. (2019). Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization. PLoS pathogens, 15(8), e1007652. |
| 55 | Ralstonia solanacearum GMI1000 | 465 | Rich mediumn; BG medium | Su, Y., Xu, Y., Li, Q., Yuan, G., & Zheng, D. (2020). The essential genome of Ralstonia solanacearum. Microbiological Research, 126500. |
| 56 | Streptococcus suis | 361 | Columbia blood base agar | Arenas, J., Zomer, A., Harders-Westerveen, J., Bootsma, H. J., De Jonge, M. I., Stockhofe-Zurwieden, N., ... & De Greeff, A. (2020). Identification of conditionally essential genes for Streptococcus suis infection in pigs. Virulence, 11(1), 446-464. |
| 57 | Mycobacterium avium subsp. hominissuis strain MAC109 | 230 | Rich medium; 7H9/10% OADC | Matern, W. M., Jenquin, R. L., Bader, J. S., & Karakousis, P. C. (2020). Identifying the essential genes of Mycobacterium avium subsp. hominissuis with Tn-Seq using a rank-based filter procedure. Scientific reports, 10(1), 1-8. |
| 58 | Providencia stuartii strain BE2467 | 496 | Rich Medium; LB medium | Johnson, A. O., et al. (2020). Transposon Insertion Site Sequencing of Providencia stuartii: Essential Genes, Fitness Factors for Catheter-Associated Urinary Tract Infection, and the Impact of Polymicrobial Infection on Fitness Requirements. Msphere, 5(3). |
| 59 | Staphylococcus aureus subsp. aureus NCTC 8325 | 288 | Rich medium; Mueller Hinton broth medium | Coe, K. A., Lee, W., Stone, M. C., Komazin-Meredith, G., Meredith, T. C., Grad, Y. H., & Walker, S. (2019). Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus. PLoS pathogens, 15(11), e1007862. |
| 60 | Staphylococcus aureus subsp. aureus USA300_TCH1516 | 295 | Rich medium; Mueller Hinton broth medium | Coe, K. A., Lee, W., Stone, M. C., Komazin-Meredith, G., Meredith, T. C., Grad, Y. H., & Walker, S. (2019). Multi-strain Tn-Seq reveals common daptomycin resistance determinants in Staphylococcus aureus. PLoS pathogens, 15(11), e1007862. |